Part:BBa_K3385041

Gene_glaA_SigP

Glucoamylase gene (glaA) with its native signal peptide.

About the part: Glucoamylase gene (glaA) with signal peptide. Predicted from the SignalP-5.0 [1].

Functionality: This signal peptide was investigated in Deeploc [2] to predict the localization of the protein which it is inserted upstream of.

Localization predicted by Deeploc.

Results: Below is a picture showing A. niger grown on starch plates with subsequent addition of iodine yielding no colouring zones showing breakdown of starch.

Starch/Iodine plate assay of the ATCC 1015 strain and a K/O (glaA) strain. A clear halo is seen were starch has been consumed since iodine binds starch.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 933
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1810
  Illegal PstI site found at 933
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 788
  Illegal BamHI site found at 1768
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 933
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 933
  Illegal NgoMIV site found at 2028
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 316
  Illegal BsaI site found at 454
  Illegal BsaI site found at 1143
  Illegal BsaI.rc site found at 1636
  

References:
[1] SignalP 5.0 improves signal peptide predictions using deep neural networks. José Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby, Thomas Nordahl Petersen, Ole Winther, Søren Brunak, Gunnar von Heijne and Henrik Nielsen. Nature Biotechnology, 37, 420-423, doi:10.1038/s41587-019-0036-z (2019)

[2] Jose Juan Almagro Armenteros, Casper Kaae Sønderby, Søren Kaae Sønderby, Henrik Nielsen, Ole Winther; DeepLoc: prediction of protein subcellular localization using deep learning, Bioinformatics, btx431